Coding
noSP OmpX

Part:BBa_K3128030:Design

Designed by: PINERO Lucas   Group: iGEM19_Grenoble-Alpes   (2019-09-23)


OmpX gene without signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Signal peptide was deleted to avoid a potential cut of the fusion protein. In deed, the signal peptide has a conformation enabling the protein addressing to the membrane. This sequence is cut after the translocation. If the OmpX gene is used without changes, the leucine zipper gene is present before the signal peptide, thus generating a cut in the fusion protein leucine-zipper-OmpX.


Source

This part comes from a synthesis of an iGEM biobrick from iGEM15_TU_Eindhoven : BBa_K1761002.


References